Journal:

Article Title: Gene Gun-Mediated DNA Immunization Primes Development of Mucosal Immunity against Bovine Herpesvirus 1 in Cattle †

doi:

Figure Lengend Snippet: MHC class II (a, b, c, and d) and AchE (e and f) staining of LCs in biopsies from nonvaccinated skin (a, b, and e) or mucosa (c, d, and f). LCs were stained with a bovine MHC class II-specific monoclonal antibody cocktail, followed by biotin-conjugated horse anti-mouse IgG and a Vectastain ABC horseradish peroxidase kit (a, b, c, and d) or with AchE (e and f). Magnifications: ×270 (a, c, e, and f) and ×810 (b and d). E, epidermis (a, b, and e) or epithelium (c, d, and f); D, dermis; L, lamina propria.

Article Snippet: Subsequently, the samples were incubated with a 1:1,000 dilution of a bovine MHC class II-specific antibody cocktail (Vector, Burlingham, Calif.) and then with a 1:5,000 dilution of biotin-conjugated horse anti-mouse IgG (Vector).

Techniques: Staining